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Mechanisms of cobalt(II) uptake into V79 Chinese hamster cells

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Mechanisms of cobalt(II) uptake into V79 Chinese hamster cells

Auteurs : Ursula Kasten [Allemagne] ; Andrea Hartwig [Allemagne] ; Detmar Beyersmann [Allemagne]

Source :

Archives of Toxicology [ 0340-5761 ] ; 1992-10-01.

RBID : ISTEX:31A1AE4232E5FFDB543B531BB70EEAEFF6A9EA06

Descripteurs français

Wicri :
- topic : Cobalt.

English descriptors

Abstract

Abstract: V79 Chinese hamster cells were used as a model for the characterization of the Co(II) uptake into mammalian cells as well as the mechanisms involved. Co(II) was taken up in a dose and time dependent manner. The uptake was exponential without saturation in the tested concentration range up to 400 μM. CoCl2. Furthermore, there was a high intracellular cobalt accumulation at elevated extra-cellular Co(II) doses (up to 16 fold at 200 μM). The time course of Co(II) uptake showed a maximum after about 8–12 h with no further change after the longest tested incubation time (24 h). The uptake of Co(II) into V79 cells seems to be mediated by multiple mechanisms: active, energy consuming transport like ion pumps and endocytosis, since the Co(II) uptake was significantly reduced by ouabain (an inhibitor of the Na+/K+ATPase), N-ethyl-maleinimide (an inhibitor of the Ca2+/Mg2+ATPase and the Na+/K+ATPase), chlorpromazine (a calmodulin antagonist and inhibitor of the Ca2+/Mg2+ ATPase) as well as by the endocytosis inhibitor chloroquine. Furthermore, the two agents iodoacetate and potassium cyanide, which produce ATP depletion, resulted in a diminution of the intracellular cobalt concentration. An uptake through anion channels could be excluded, since 4,4′-diisothiocyanostilbene-2,2′-disulphonic acid was not inhibitory

Url:

https://api.istex.fr/ark:/67375/1BB-69N7HSJN-7/fulltext.pdf

DOI: 10.1007/BF01973391

Affiliations:

Allemagne

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract: V79 Chinese hamster cells were used as a model for the characterization of the Co(II) uptake into mammalian cells as well as the mechanisms involved. Co(II) was taken up in a dose and time dependent manner. The uptake was exponential without saturation in the tested concentration range up to 400 μM. CoCl2. Furthermore, there was a high intracellular cobalt accumulation at elevated extra-cellular Co(II) doses (up to 16 fold at 200 μM). The time course of Co(II) uptake showed a maximum after about 8–12 h with no further change after the longest tested incubation time (24 h). The uptake of Co(II) into V79 cells seems to be mediated by multiple mechanisms: active, energy consuming transport like ion pumps and endocytosis, since the Co(II) uptake was significantly reduced by ouabain (an inhibitor of the Na+/K+ATPase), N-ethyl-maleinimide (an inhibitor of the Ca2+/Mg2+ATPase and the Na+/K+ATPase), chlorpromazine (a calmodulin antagonist and inhibitor of the Ca2+/Mg2+ ATPase) as well as by the endocytosis inhibitor chloroquine. Furthermore, the two agents iodoacetate and potassium cyanide, which produce ATP depletion, resulted in a diminution of the intracellular cobalt concentration. An uptake through anion channels could be excluded, since 4,4′-diisothiocyanostilbene-2,2′-disulphonic acid was not inhibitory</div>
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Mechanisms of cobalt(II) uptake into V79 Chinese hamster cells

Mechanisms of cobalt(II) uptake into V79 Chinese hamster cells

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri